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当前位置：水资源研究与管理 > 2025年 1卷 (1)期

Open Access Article

Water Resources Research and Management. 2025; 1: (1) ; 11-17 ; DOI: 10.12208/j.wrrm.20250003.

Research on the Influence of Inoculating Bacterium Suspension on Microbial Community Structure in Petroleum Hydrocarbon Contaminated Groundwater
石油烃污染地下水中微生物群落结构的影响研究

作者： Xueyang Huang^1,2, Guo Wei³, Ziting Ding⁴, Huazhi Wang¹, Liping Bai¹ *

1 生态环境部土壤与农业与农村生态环境技术中心北京

2 中国地质大学水资源与环境学院北京

3 北京投资集团有限公司北京

4 山西坤宏环保科技有限公司山西太原

*通讯作者： Liping Bai,单位：生态环境部土壤与农业与农村生态环境技术中心北京；

国家自然科学基金（No.41471266）

发布时间: 2025-04-15 总浏览量: 239

PDF 全文下载引用本文

摘要

石油烃是通过采集北京某石油烃污染场地土壤样品筛选分离降解菌，将培养的菌悬液按不同比例添加到地下水样中，分析不同比例菌悬液对地下水样中细菌群落结构的影响，研究可利用石油污染物作为碳源和能量来源的细菌。研究结果表明，菌悬液的添加对地下水样中细菌结构有明显影响，与受石油烃污染的原水样相比，24 h后变形菌门和厚壁菌门的相对丰度分别由88%提高到97%和由0.4%提高到34.2%。24h后假单胞菌属和芽孢杆菌属相对丰度分别由67%上升至99%和0.2%上升至32.3%，地下水样品中的优势菌为石油烃相关降解菌，研究结果可为石油污染土壤及地下水的生物修复提供理论依据。

关键词： 污染场地；地下水；石油烃；降解菌

Abstract

Petroleum hydrocarbon is a common pollutant which has high toxicity and transport property in groundwater. Considering the current supervision demands of groundwater pollution in China, this paper studies the bioremediation method of petroleum hydrocarbon contaminated groundwater. The degradation bacteria were screened and separated through the soil samples collected at a petroleum hydrocarbon contaminated site in Beijing, China. The cultivated bacterial suspension was added by different proportions into the groundwater samples. The influence of different proportions of bacteria suspension on the bacteria community structure in groundwater samples was analyzed, and the bacteria which could use petroleum pollutants as carbon sources and energy sources were studied. The research results showed that addition of bacterial suspension could have significantly effects on the bacterial structure in groundwater sample. Compared with the raw water samples contaminated by petroleum hydrocarbons, the relative abundance of Proteobacteria and Firmicutes phylum level increased respectively from 88% to 97% and 0.4% to 34.2% after 24 hours. The relative abundance of Pseudomonas and Bacillus genus level increased respectively from 67% to 99%, and 0.2% to 32.3% after 24 hours. The advantage bacteria in groundwater samples were considered as petroleum hydrocarbon-related degradation bacteria. The research results could provide theoretical basis for the bioremediation of petroleum contaminated soil and groundwater.

Key words： Contaminated site; Groundwater; Petroleum hydrocarbon; Degradation bacteria

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引用本文

XueyangHuang, GuoWei, ZitingDing, HuazhiWang, LipingBai, 石油烃污染地下水中微生物群落结构的影响研究[J]. 水资源研究与管理, 2025; 1: (1) : 11-17.

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